Nucleoid-associated Rok differentially affects chromosomal transformation on Bacillus subtilis recombination-deficient cells

Ester Serrano et al. Environ Microbiol. 2021 Jun.

Abstract

Rok, a Bacillus subtilis nucleoid-associated protein (NAP), negatively regulates competence development and silences xenogeneic genes. We show that rok inactivation increases rpoB482 natural intraspecies chromosomal transformation (CT) and plasmid transformation vĩ đại a different extent. In ΔaddAB, ΔrecO, recF15, ΔrecU, ΔruvAB or rec⁺ cells intraspecies CT significantly increases, but the ΔrecD2 mutation reduces, and the ΔrecX, ΔradA or ΔdprA mutation further decreases CT in the Δrok context when compared vĩ đại rok⁺ cells. These observations tư vấn the idea that rok inactivation, by altering the topology of the recipient DNA, differentially affects the integration of homologous DNA in rec-deficient strains, and in minor extent the competent subpopulation size. The impairment of other NAP (Hbsu or LrpC) also increased intra- and interspecies CT (nonself-DNA, ~8% nucleotide sequence divergence) in rec⁺ cells, but differentially reduced both types of CTs in certain rec-deficient strains. We describe that rok inactivation significantly stimulates intra and interspecies CT but differentially reduces them in transformation-deficient cells, perhaps by altering the nucleoid architecture. We extend the observation vĩ đại other NAPs (Hbsu, LrpC).

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References

1. Albano, M., Smits, W.K., Ho, L.T., Kraigher, B., Mandic-Mulec, I., Kuipers, O.P., and Dubnau, D. (2005) The Rok protein of Bacillus subtilis represses genes for cell surface and extracellular functions. J Bacteriol 187: 2010-2019.
1. Ali, S.S., Soo, J., Rao, C., Leung, A.S., Ngai, D.H., Ensminger, A.W., and Navarre, W.W. (2014) Silencing by H-NS potentiated the evolution of Salmonella. PLoS Pathog 10: e1004500.
1. Alonso, J.C., Tailor, R.H., and Luder, G. (1988) Characterization of recombination-deficient mutants of Bacillus subtilis. J Bacteriol 170: 3001-3007.
1. Alonso, J.C., Luder, G., and Tailor, R.H. (1991) Characterization of Bacillus subtilis recombinational pathways. J Bacteriol 173: 3977-3980.
1. Azam, T.A., and Ishihama, A. (1999) Twelve species of the nucleoid-associated protein from Escherichia coli. Sequence recognition specificity and DNA binding affinity. J Biol Chem 274: 33105-33113.